Cultivable Microbiota in Mitis Salivarius Agar from Dental Plaque of Dogs
Fábio Alessandro Pieri,
Vitor de Oliveira Silva,
Abelardo Silva Junior,
Maria Aparecida Scatamburlo Moreira
Issue:
Volume 6, Issue 2, March 2018
Pages:
21-26
Received:
6 April 2018
Accepted:
23 April 2018
Published:
15 May 2018
Abstract: Periodontal disease, which affects the support structures and protection of teeth, is the main disease found in dogs, reaching about 85% prevalence in animals over 4 years old. The most important bacteria in the initial colonization of human dental plaque are Streptococcus, which produce exopolysaccharides that facilitate fixation of the bacteria to the surfaces. Some studies have sought to identify the dental plaque microbiota in dogs using non-selective culture media, and have reported small amounts of Streptococcus, however they did not provide ideal conditions for the growth of this bacteria. The aim of this study was to isolate and identify the initial dental plaque microbiota of dogs cultivable in a selective medium for Streptococcus, and verify the presence of this genus. Genotypic identification of the isolates were carried out by partial 16S rDNA sequencing. A total of 179 strains were obtained from the plaque of 10 dogs without periodontal disease. The technique employed allowed 15 different microbial groups to be isolated, including the genus Streptococcus. This represented 33 (18.4%) of 179 isolates, thus illustrating the partially selective ability of Mitis Salivarius Agar for samples of dental plaque in dogs.
Abstract: Periodontal disease, which affects the support structures and protection of teeth, is the main disease found in dogs, reaching about 85% prevalence in animals over 4 years old. The most important bacteria in the initial colonization of human dental plaque are Streptococcus, which produce exopolysaccharides that facilitate fixation of the bacteria t...
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Antioxidant Enzymes and Lipid Peroxidation in Alloxan-Induced Diabetic Rabbits
Enefe Ndidi Glory,
Ebuehi Osaretin Albert
Issue:
Volume 6, Issue 2, March 2018
Pages:
27-34
Received:
20 April 2018
Accepted:
17 May 2018
Published:
15 June 2018
Abstract: The study was carried out to evaluate the effect of alloxan–induced diabetes on some oxidative stress markers in rabbits. Ten rabbits weighing between 1.0-1.5kg were used and divided into two groups of five rabbits each. Diabetes was induced in one of the groups, by intraperitonial administration of alloxan at a dosage of 150mg/kg body weight and the control rabbits were administered normal saline (0.9% NaCl). The animals were monitored for 14days after confirmed diabetes in the test group. The fasting plasma glucose, plasma insulin, plasma lipids, the antioxidant enzymes: Superoxide dismutase (SOD), catalase, and lipid peroxidation were assayed for, in the plasma and the brain of the diabetic and non-diabetic rabbits. The results showed a significant decrease in mean body weight of the alloxan-induced diabetic and control rabbits pre and 14days post alloxan-induction. There was a significant (p < 0.01) increase in fasting plasma glucose, a significant (p < 0.01) decrease in the activity of SOD and a significant (p < 0.01) increase in the activity of catalase in the plasma and brain; there was also a significant (p < 0.01) increase in the level of lipid peroxidation, plasma cholesterol and triglycerides in the diabetic rabbits. Culminating a depletion of SOD activity, the altered activity of plasma (or brain) catalase and increased lipid peroxidation is due to the oxidative stress induced by the diabetic condition, hence the present study indicate that alloxan – induced diabetic potentiated oxidative stress in a short term duration. This shows the importance of antioxidant mimetics to be administered at early stage of diabetics in other to increase the antioxidant capacity thus preventing oxidative stress which may lead to complication in diabetes.
Abstract: The study was carried out to evaluate the effect of alloxan–induced diabetes on some oxidative stress markers in rabbits. Ten rabbits weighing between 1.0-1.5kg were used and divided into two groups of five rabbits each. Diabetes was induced in one of the groups, by intraperitonial administration of alloxan at a dosage of 150mg/kg body weight and t...
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