Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey
Issue:
Volume 4, Issue 1, January 2016
Pages:
1-4
Received:
8 December 2015
Accepted:
16 December 2015
Published:
30 December 2015
Abstract: Rotavirus is the most common cause of severe diarrhea in newborn calves. In this study, it was aimed to investigate frequency of Rotavirus infections in calves in Kars region in Northeast Turkey by feces sampling 112 diarrheic calves of 0-6 months old. Feces samples were tested for Rotavirus antigen by Enzyme Linked Immunosorbent Assay (ELISA). Of the 112 feces samples examined by ELISA 10 samples were identified as positive (8.92%). At the molecular stage, reverse transcription polymerase chain reaction (RT-PCR) technique was used to determine the presence of Rotavirus nucleic acid by using specific generic primers for VP6 genetic regions in feces samples. The 10 samples that were rotavirus antigen positive by ELISA were also positive on RT-PCR technique. The proportion of positivity in feces samples suggested that rotavirus infections are common in the Kars region in Northeast Turkey.
Abstract: Rotavirus is the most common cause of severe diarrhea in newborn calves. In this study, it was aimed to investigate frequency of Rotavirus infections in calves in Kars region in Northeast Turkey by feces sampling 112 diarrheic calves of 0-6 months old. Feces samples were tested for Rotavirus antigen by Enzyme Linked Immunosorbent Assay (ELISA). Of ...
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Effect of Traditional Cattle Farming Systems on Farmer Knowledge, Cattle Performances and Agribusiness Potential in West New Guinea-Papua Barat Province, Indonesia
Deny A. Iyai,
Desni T. R. Saragih,
Frandz P. Rumbiak
Issue:
Volume 4, Issue 1, January 2016
Pages:
5-10
Received:
4 November 2015
Accepted:
13 November 2015
Published:
1 February 2016
Abstract: Cattle have been long time introduced in Papua. However adaptation to raise cattle has been unpredictable. An explorative field study using participatory analysis was done in fifteen villages to comprehensively understand the dynamic of cattle performances. Cattle farming system was classified in to three sizes, i.e. small-medium- and large systems. Analyses of data were using Anova and Kruskal-Wallis. The finding of this research was that the three cattle farming sizes were similar in many components of good agricultural practices (p>0.05). No wide gap was found in line with farmers, cattle and economical performances. Tropical livestock unit of West New Guinea was low and has possibility to be increased due to agribusiness potential. Lack of good agricultural practices should be narrowed down by better services of government and other related stakeholders.
Abstract: Cattle have been long time introduced in Papua. However adaptation to raise cattle has been unpredictable. An explorative field study using participatory analysis was done in fifteen villages to comprehensively understand the dynamic of cattle performances. Cattle farming system was classified in to three sizes, i.e. small-medium- and large systems...
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Diet-Dependent Immunohistochemical Evaluation of Connexin 43 in the Sheep Rumen
Rasha S. Ahmed,
Holger Martens,
Christoph Muelling
Issue:
Volume 4, Issue 1, January 2016
Pages:
11-14
Received:
28 January 2016
Accepted:
8 February 2016
Published:
26 February 2016
Abstract: The objective of this study was to characterize the immunohistochemical localization of plasma membrane connexin 43 in the rumen of sheep after changing the diet from hay (ad libitum) to a mixed hay/concentrate diet. A total of 24 sheep were fed mixed hay/concentrate for different periods ranging from 0 weeks (control; hay ad libitum) to 12 weeks (1-1.5 kg hay plus 780 g concentrate per day in two equal portions). Using immunohistochemical technique the present study confirmed the existence of plasma membrane connexin 43 in the sheep rumen epithelium. Plasma membrane connexin 43 immunostaining was most intense at the stratum basale and stratum spinosum (suprabasal layer) and decreased iron intensity through stratum spinosum (superficial layers) to stratum granulosum. Meanwhile, stratum corneum was negative. The reaction around the cells gave a syncitial appearance with more apical-immunostaining concentration. Moreover, the present study confirmed a significant effect of concentrate diet on the immunoreactivity of plasma membrane connexin 43 in the rumen of sheep. A very strong degree of antibody reaction was seen in 4 to 12 weeks concentrate-fed groups.
Abstract: The objective of this study was to characterize the immunohistochemical localization of plasma membrane connexin 43 in the rumen of sheep after changing the diet from hay (ad libitum) to a mixed hay/concentrate diet. A total of 24 sheep were fed mixed hay/concentrate for different periods ranging from 0 weeks (control; hay ad libitum) to 12 weeks (...
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