Ethological Problems and Learning Disability Due to Aluminum Toxicity in Rats
Amira A. Goma,
Usama E. Mahrous
Issue:
Volume 1, Issue 2, March 2013
Pages:
12-17
Abstract: A total of 35 Sprague-Dawley adult rats were used to investigate the effect of aluminum toxicity on behavioral patterns of adult female rats and learning ability of offspring. Rats were allotted into 4 groups, group one received 2g/l anhydrous aluminum chloride (n=10), group two received 3g/l anhydrous aluminum chloride (n=10), group thr.ee received 3.5g/l anhydrous aluminum chloride in drinking water (n=10) and control group did not receive anhydrous aluminum chloride (n=5) from 8th day of pregnancy till weaning of pups. The obtained results showed that feeding time increased significantly in 2g/l and 3.5g/l anhydrous aluminum chloride groups than control one, while, litter licking frequency and nursing time increased significantly in 2g/l anhydrous aluminum chloride than other groups. On contrary lying time decreased significantly in rats treated with 2g/l anhydrous aluminum chloride than other groups, licking and scratching decreased in 3g/l and 3.5g/l anhydrous aluminum chloride groups. In considering, the time spent in closed arms by offspring, pups spent significantly much time than control group, while, time spent in open arms of elevated plus maze decreased significantly in all treated groups than control group. On the other hand, number of entries in open arms significantly decreased in treated groups than control one.
Abstract: A total of 35 Sprague-Dawley adult rats were used to investigate the effect of aluminum toxicity on behavioral patterns of adult female rats and learning ability of offspring. Rats were allotted into 4 groups, group one received 2g/l anhydrous aluminum chloride (n=10), group two received 3g/l anhydrous aluminum chloride (n=10), group thr.ee receive...
Show More
Assessment of Transaminases and Effect of Freezing Rates on Their Leakage into Seminal Plasma of Sirohi Bucks
Sunanda Sharma,
Narendra K. Sharma,
N. K. Sinha,
S. S. Sharma
Issue:
Volume 1, Issue 2, March 2013
Pages:
18-22
Abstract: Seminal plasma transaminases (GOT and GPT) were assessed in diluted (Tris-egg yolk-citric acid-fructose-glycerol extender 1:4) and thawed (400C for 15 seconds) semen of Sirohi bucks (control group). Diluted, cooled (from 250C up to 50C) and equilibrated (at 50C for 2 hours) semen straws were frozen @ 15, 20, 25 and 300C/minute for M1, M2, M3 and M4 groups respectively. These frozen straws were stored separately in LN2. Effect of freezing rates on leakage of tran-ferases was studied by assessing GOT and GPT in seminal plasma of thawed semen from M1, M2, M3 and M4 groups. The Mean + S.E. (C.V.%) values of transferases (GOT : GPT) in M1, M2, M3, M4 and control group were 313.00 + 8.40 (6.58) : 20.5 + 0.43 (5.12), 272.5 + 6.68 (6.01) : 16.33 + 1.02 (15.33), 255.00 + 10.1 (9.7) : 14.5 + 0.67 (11.33), 292.0 + 9.67 (8.09) : 19.67 + 0.70 (8.99) and 147.67 + 10.96 (18.17) ; 9.5 + 0.62 (15.96) units per 0.92 x 109 spermatozoa respectively. Least square analysis of variance revealed highly significant (P < 0.01) rise in the seminal plasma GOT enzyme levels in frozen thawed semen as compared to that in fresh diluted, cooled and equilibrated semen. Among the freezing mode groups lowest GOT and GPT values in seminal plasma was observed in M3 followed by M2, M4 and M1 groups in increasing order.
Abstract: Seminal plasma transaminases (GOT and GPT) were assessed in diluted (Tris-egg yolk-citric acid-fructose-glycerol extender 1:4) and thawed (400C for 15 seconds) semen of Sirohi bucks (control group). Diluted, cooled (from 250C up to 50C) and equilibrated (at 50C for 2 hours) semen straws were frozen @ 15, 20, 25 and 300C/minute for M1, M2, M3 and M4...
Show More
