Volume 8, Issue 4, July 2020, Page: 93-95
Platelet and Transforming Growth Factor Beta 1 Concentrations in Platelet-rich Plasma in Dogs
Inmaculada Peris, Department of Animal Medicine and Surgery, Faculty of Veterinary and Experimental Sciences, Catholic University of Valencia “San Vicente Martir”, Valencia, Spain
Blanca Esquembre, Department of Animal Medicine and Surgery, Faculty of Veterinary and Experimental Sciences, Catholic University of Valencia “San Vicente Martir”, Valencia, Spain
Jose Ignacio Redondo, Faculty of Veterinary, CEU – Cardenal Herrera University, CEU Universities, Valencia, Spain
Vicente Jose Sifre, Department of Animal Medicine and Surgery, Faculty of Veterinary and Experimental Sciences, Catholic University of Valencia “San Vicente Martir”, Valencia, Spain
Carme Soler, Department of Animal Medicine and Surgery, Faculty of Veterinary and Experimental Sciences, Catholic University of Valencia “San Vicente Martir”, Valencia, Spain
Claudio Ivan Serra, Department of Animal Medicine and Surgery, Faculty of Veterinary and Experimental Sciences, Catholic University of Valencia “San Vicente Martir”, Valencia, Spain
Received: Jul. 20, 2020;       Accepted: Aug. 5, 2020;       Published: Aug. 19, 2020
DOI: 10.11648/j.avs.20200804.15      View  67      Downloads  35
Abstract
Platelet-rich plasma showed good results in tissue healing when first used in human medicine. After that, its use spread to veterinary medicine. However, there is no standardized method for manual collection of platelet – rich plasma in the canine species. The objectives of this study were to standardize a protocol to obtain platelet – rich plasma (PRP) with high concentration of platelets and transforming growth factor β1 (TGF – β1) without the presence of erythrocytes and leukocytes; and to relate the presence of TGF – β1 with the amount of platelets. For this purpose, there were obtained two blood samples separated one week between each other, from eleven healthy Beagles. Blood samples were centrifuged using different protocols: protocol A (one centrifuge, 210 g and 10 minutes), protocol B (double centrifuge, first one 210 g and 10 minutes, second one 210 g and 15 minutes) and protocol C (one centrifuge, 475 g and 8 minutes). Three plasma fractions were obtained through these protocols: a platelet-rich fraction, a platelet-poor fraction and whole blood. The content of leukocytes, erythrocytes and platelets was measured in the whole blood and plasma rich and poor fractions of the protocol A, B and C. The TGF – β1 concentration was measured in the platelet rich and poor fraction of A and B protocols. The results showed a higher concentration of platelets and TGF – β1 in protocol A. In conclusion, this study offers an economical and reproducible method for obtaining PRP in the canine specie.
Keywords
Dog, Platelet, PRP, Growth Factor, TGF – β1
To cite this article
Inmaculada Peris, Blanca Esquembre, Jose Ignacio Redondo, Vicente Jose Sifre, Carme Soler, Claudio Ivan Serra, Platelet and Transforming Growth Factor Beta 1 Concentrations in Platelet-rich Plasma in Dogs, Animal and Veterinary Sciences. Vol. 8, No. 4, 2020, pp. 93-95. doi: 10.11648/j.avs.20200804.15
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
Carr BJ, Canapp Jr SO, Mason DR, Cox T, Hess T. Canine Platelet-Rich Plasma Systems: A Prospective Analysis. Front Vet Sci 2016; 2: 73.
[2]
Marx RE, Carlson ER, Eichstaedt RM, Schimmel SR, Strauss JE, Georgeff KR. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998; 85 (6): 638-46.
[3]
Anitua E, Sánchez M, Orive G, Andía I. The potential impact of the preparation rich in growth factors (PRGF) in different medical fields. Biomaterials 2007; 28 (31): 4551-60.
[4]
Karayannopoulou M, Papazoglou LG, Loukopoulos P, et al. Locally injected autologous platelet-rich plasma enhanced tissue perfusion and improved survival of long subdermal plexus skin flaps in dogs. Vet Comp Orthop Traumatol 2014; 4; 27 (5).
[5]
Rabillard M, Grand J-G, Delibert E, Fellah B, Gauthier O, Niebauer GW. Effect of autologous platelet rich plasma gel and calcium phosphate biomaterials on bone healing in an ulnar ostectomy model in dogs. Vet Comp Orthop Traumatol 2009; 22 (6): 460-6.
[6]
Jee CH, Eom NY, Jang HM, et al. Effect of autologous platelet-rich plasma application on cutaneous wound healing in dogs. J Vet Sci 2016; 17 (1): 79–87.
[7]
Fahie MA, Ortolano GA, Guercio V, et al. A randomized controlled trial of the efficacy of autologous platelet therapy for the treatment of osteoarthritis in dogs. JAVMA 2013; 243 (9): 1291-1297.
[8]
Hilty DH, Yocum JG Y, Gallichio VS. Examining the Effectiveness of Stem Cell and Platelet-Rich Plasma Therapies for Treating Osteoarthritis in Dogs. Anim Husb Dairy Vet Sci 2017; 1 (4).
[9]
Silva RF, Carmona JU, Rezende CMF. Intra-articular injections of autologous platelet concentrates in dogs with surgical reparation of cranial cruciate ligament rupture. Veterinary and Comparative Orthopaedics and Traumatology 2013; 26 (4): 285-290.
[10]
Magalon J, Bausset O, Serratrice N, et al. Characterization and comparison of 5 platelet-rich plasma preparations in a single-donor model. Arthroscopy 2014; 30 (5): 629-638.
[11]
Stief M, Gottschalk J, Ionita J-C, Einspanier A, Oechtering G, Böttcher P. Concentration of platelets and growth factors in canine autologous conditioned plasma. Vet Comp Orthop Traumatol 2011; 24 (2): 122-5.
[12]
Shin HS, Woo HM, Kang BJ. Optimisation of a double-centrifugation method for preparation of canine platelet-rich plasma. BMC Vet Res 2017; 13 (1): 198.
[13]
Casati MZ, De Vasconcelos Gurgel BC, Gonçalves PF, et al. Platelet-rich plasma does not improve bone regeneration around peri-implant bone defects--a pilot study in dogs. Int J Oral Maxillofac Surg 2007; 36 (2): 132-6.
[14]
De Vasconcelos Gurgel BC, Goncalves PF, Pimentel SP, et al. Platelet-rich plasma may not provide any additional effect when associated with guided bone regeneration around dental implants in dogs. Clin Oral Implants Res 2007; 18 (5): 649-54.
[15]
Anitua E, Andia I, Ardanza B, Nurden P, Nurden AT. Autologous platelets as a source of proteins for healing and tissue regeneration. Throm Haemost 2004; 91 (1): 4-15.
[16]
Okuda K, Kawase T, Momose M, et al. Platelet-rich plasma contains high levels of platelet-derived growth factor and transforming growth factor-beta and modulates the proliferation of periodontally related cells in vitro. J Periodontol 2003; 74 (6): 849-57.
[17]
Ouyang XY and Qiao J. Effect of platelet-rich plasma in the treatment of periodontal intrabony defects in humans. Chin Med J (Engl) 2006; 119 (18): 1511-21.
[18]
Carmona JU, Argüelles D, Climent F, Prades M. Autologous platelet concentrates as a treatment of horses with osteoarthritis: A preliminary pilot clinical study. Journal of Equine Veterinary Science 2007; 27: 167-170.
[19]
Grageda E, Lozada JL, Boyne PJ, Caplanis N, McMillian PJ. Bone formation in the maxillary sinus by using platelet-rich plasma: an experimental study in sheep. J Oral Implantol 2005; 31 (1): 2-17.
[20]
Wehling P, Moser C, Frisbie D, et al. Autologous conditioned serum in the treatment of orthopedic diseases: the orthokine therapy. BioDrugs 2007; 21 (5): 323-32.
[21]
Weibrich G, Kleis WK, Hitzler WE, Hafner G. Comparison of the platelet concentrate collection system with the plasma-rich-in-growth-factors kit to produce platelet-rich plasma: a technical report. Int J Oral Maxillofac Implants 2005; 20 (1): 118-23.
[22]
Franklin SP, Garner BC, Cook JL. Characteristics of canine platelet-rich plasma prepared with five commercially available systems. American journal of veterinary research 2015; 76 (9): 822-827.
[23]
Nagata MJ, Messora MR, Furlaneto FA, et al. Effectiveness of two methods for preparation of autologous platelet-rich plasma: an experimental study in rabbits. European journal of dentistry 2010; 4 (4): 395-402.
[24]
Mazzocca AD, McCarthy MBR, Chowaniec DM, et al. Platelet-rich plasma differs according to preparation method and human variability. JBJS 2012; 94 (4): 308-316.
[25]
Serra CI, Soler C, Carrillo JM, Sopena JJ, Redondo JI, Cugat R. Effect of autologous platelet – rich plasma on the repair of full – thickness articular deffects in rabbit. Knee Surg Sports Traumatol Arthrosc 2013; 21: 1730-1736.
[26]
Araki J, Jona M, Eto H, et al. Optimized preparation method of platelet-concentrated plasma and noncoagulating platelet-derived factor concentrates: maximization of platelet concentration and removal of fibrinogen. Tissue Eng Part C Methods 2012; 18 (3): 176–185.
[27]
Amable PR, Carias RBV, Teixeira MVT, et al. Platelet-rich plasma preparation for regenerative medicine: optimization and quantification of cytokines and growth factors. Stem cell research & therapy 2013; 4 (3): 67.
[28]
Monobe MM. Canine pure platelet-rich plasma for regenerative medicine and platelet research: protocol optimization [PhD Thesis]. Meridian, Mississippi: Mississippi State University, 2016.
[29]
Chopra R and Anastassiades T. Specificity and synergism of polypeptide growth factors in stimulating the synthesis of proteoglycans and a novel high molecular weight anionic glycoprotein by articular chondrocyte cultures. J Rheumatol 1998; 25 (8): 1578-84.
[30]
Gerard D, Carlson ER, Gotcher JE, Jacobs M. Effects of platelet-rich plasma on the healing of autologous bone grafted mandibular defects in dogs. J Oral Maxillofac Surg 2006; 64 (3): 443-51.
[31]
Marx RE. The biology of platelet-rich plasma. Journal of Oral Maxillofacial Surgery 2001; 59: 1119-1121.
[32]
Weibrich G, Hansen T, Kleis W, Buch R, Hitzler WE. Effect of platelet concentration in platelet-rich plasma on peri-implant bone regeneration. Bone 2004; 34 (4): 665-71.
[33]
Gleizes PE, Munger JS, Nunes I, et al. TGF-beta latency: biological significance and mechanisms of activation. Stem Cells 1997; 15 (3): 190-7.
[34]
Franklin SP, Birdwhistell KE. Assessment of Canine Autologous Conditioned PlasmaTM Cellular and Transforming Growth Factor-β1 Content. Front Vet Sci 2018; 5: 105.
[35]
Assoian RK, Komoriya A, Meyers CA, Miller DM, Sporn MB. Transforming growth factor-beta in human platelets. Identification of a major storage site, purification, and characterization. J Biol Chem 1983; 258 (11): 7155-60.
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