Volume 8, Issue 4, July 2020, Page: 93-95
Platelet and Transforming Growth Factor Beta 1 Concentrations in Platelet-rich Plasma in Dogs
Inmaculada Peris, Department of Animal Medicine and Surgery, Faculty of Veterinary and Experimental Sciences, Catholic University of Valencia “San Vicente Martir”, Valencia, Spain
Blanca Esquembre, Department of Animal Medicine and Surgery, Faculty of Veterinary and Experimental Sciences, Catholic University of Valencia “San Vicente Martir”, Valencia, Spain
Jose Ignacio Redondo, Faculty of Veterinary, CEU – Cardenal Herrera University, CEU Universities, Valencia, Spain
Vicente Jose Sifre, Department of Animal Medicine and Surgery, Faculty of Veterinary and Experimental Sciences, Catholic University of Valencia “San Vicente Martir”, Valencia, Spain
Carme Soler, Department of Animal Medicine and Surgery, Faculty of Veterinary and Experimental Sciences, Catholic University of Valencia “San Vicente Martir”, Valencia, Spain
Claudio Ivan Serra, Department of Animal Medicine and Surgery, Faculty of Veterinary and Experimental Sciences, Catholic University of Valencia “San Vicente Martir”, Valencia, Spain
Received: Jul. 20, 2020;       Accepted: Aug. 5, 2020;       Published: Aug. 19, 2020
DOI: 10.11648/j.avs.20200804.15      View  67      Downloads  35
Platelet-rich plasma showed good results in tissue healing when first used in human medicine. After that, its use spread to veterinary medicine. However, there is no standardized method for manual collection of platelet – rich plasma in the canine species. The objectives of this study were to standardize a protocol to obtain platelet – rich plasma (PRP) with high concentration of platelets and transforming growth factor β1 (TGF – β1) without the presence of erythrocytes and leukocytes; and to relate the presence of TGF – β1 with the amount of platelets. For this purpose, there were obtained two blood samples separated one week between each other, from eleven healthy Beagles. Blood samples were centrifuged using different protocols: protocol A (one centrifuge, 210 g and 10 minutes), protocol B (double centrifuge, first one 210 g and 10 minutes, second one 210 g and 15 minutes) and protocol C (one centrifuge, 475 g and 8 minutes). Three plasma fractions were obtained through these protocols: a platelet-rich fraction, a platelet-poor fraction and whole blood. The content of leukocytes, erythrocytes and platelets was measured in the whole blood and plasma rich and poor fractions of the protocol A, B and C. The TGF – β1 concentration was measured in the platelet rich and poor fraction of A and B protocols. The results showed a higher concentration of platelets and TGF – β1 in protocol A. In conclusion, this study offers an economical and reproducible method for obtaining PRP in the canine specie.
Dog, Platelet, PRP, Growth Factor, TGF – β1
To cite this article
Inmaculada Peris, Blanca Esquembre, Jose Ignacio Redondo, Vicente Jose Sifre, Carme Soler, Claudio Ivan Serra, Platelet and Transforming Growth Factor Beta 1 Concentrations in Platelet-rich Plasma in Dogs, Animal and Veterinary Sciences. Vol. 8, No. 4, 2020, pp. 93-95. doi: 10.11648/j.avs.20200804.15
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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