Volume 7, Issue 2, March 2019, Page: 59-68
Evaluation of Viral Migration of Different Variants of Equid Alphaherpesvirus 1 in the Central Nervous System of Hamsters by Immunohistochemistry
Aline Aparecida da Silva, Biological Institute, Avenida Conselheiro Rodrigues Alves, São Paulo/SP, Brasil
Elenice Maria Sequetin Cunha, Biological Institute, Avenida Conselheiro Rodrigues Alves, São Paulo/SP, Brasil
Maria do Carmo Custódio de Souza Hunold Lara, Biological Institute, Avenida Conselheiro Rodrigues Alves, São Paulo/SP, Brasil
Eliana Monteforte Cassaro Villalobos, Biological Institute, Avenida Conselheiro Rodrigues Alves, São Paulo/SP, Brasil
Alessandra Figueiredo de Castro Nassar, Biological Institute, Avenida Conselheiro Rodrigues Alves, São Paulo/SP, Brasil
Enio Mori, Pasteur Institute, Avenida Paulista, São Paulo/SP, Brasil
Carolina Natalia Zanuzzi, Department of Histology and Embryology, Faculty of Veterinary Sciences, National University of La Plata, La Plata, Buenos Aires, Argentina
Cecília Mónica Galosi, Department of Histology and Embryology, Faculty of Veterinary Sciences, National University of La Plata, La Plata, Buenos Aires, Argentina
Claudia Del Fava, Biological Institute, Avenida Conselheiro Rodrigues Alves, São Paulo/SP, Brasil
Received: Mar. 12, 2019;       Accepted: Apr. 12, 2019;       Published: Jun. 13, 2019
DOI: 10.11648/j.avs.20190702.15      View  204      Downloads  46
Abstract
The intranasal inoculation of equid alphaherpesvirus1 (EHV-1) Brazilian variants A4/72, A9/92, A3/97, Iso72/10 and the Argentine variant AR4 in a Syrian hamster model Mesocricetus auratus induced severe encephalitis. Clinical signs included weight loss, lethargy, somnolence, anorexia, and intense salivation two days post-inoculation (dpi), followed by neurological signs such as loss of proprioception, walking in circles, spastic paralysis, seizures, recumbency and death at 3rd dpi (A9/92 and A4/72 variants) and 4th dpi. Respiratory signs such as dyspnea and serosanguinous nasal discharge were also observed. Histopathological changes in brain included mixed inflammatory infiltrate with predominance of mononuclear cells, neuronal degeneration, liquefactive necrosis, hemorrhagic foci, leptomeningitis, perivascular edema, mononuclear infiltration, and perivascular cuffing. Immunohistochemical examination showed viral replication in neurons restrict predominantly to olfactory bulb and frontal cortex (variants AR4 and A3/97) and in groups of cells from distant regions, such as the caudal diencephalon and rostral mesencephalon (variants Iso72/10) and absence of viral antigen labeling of variants A9/92 and A4/72 despite these variants were the most neurovirulent, so new experiments not staggered in days but in hours post inoculation are needed to better understand the viral migration of these variants.
Keywords
EHV-1, Neurovirulence, Neuropathogenicity, Immunohistochemistry, Myeloencephalopathy
To cite this article
Aline Aparecida da Silva, Elenice Maria Sequetin Cunha, Maria do Carmo Custódio de Souza Hunold Lara, Eliana Monteforte Cassaro Villalobos, Alessandra Figueiredo de Castro Nassar, Enio Mori, Carolina Natalia Zanuzzi, Cecília Mónica Galosi, Claudia Del Fava, Evaluation of Viral Migration of Different Variants of Equid Alphaherpesvirus 1 in the Central Nervous System of Hamsters by Immunohistochemistry, Animal and Veterinary Sciences. Vol. 7, No. 2, 2019, pp. 59-68. doi: 10.11648/j.avs.20190702.15
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Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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