Volume 6, Issue 4, July 2018, Page: 51-57
Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (Acipenser schrenckii)
Xiujuan Zhang, Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, China
Jinge Ma, Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, China
Linmiao Li, Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, China
Haiying Jiang, Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, China
Jinping Chen, Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, China
Received: Aug. 4, 2018;       Accepted: Sep. 4, 2018;       Published: Oct. 13, 2018
DOI: 10.11648/j.avs.20180604.11      View  396      Downloads  8
Abstract
Development of artificial propagation technology is becoming increasingly important in sturgeon aquaculture whether species recovery efforts or commercial production. The cryopreservation technique of high-quality semen collected during the spawning season could extremely improve reproductive efficiency for year-round availability, especially for off-season use. However, the cryopreservation technique of sperm in Amur sturgeon (Acipenser schrenckii) has not been effectively developed. In the present study, we firstly tested the cryopreservations combination effects of three cryoprotectants, including methanol, dimethyl sulfoxide (DMSO), and propylene glycol (PG) and fresh yolk (Y) addition on sperm motility, fertilization and embryo development in Amur sturgeon. The results indicated that sperm motility was still more than 60% in the MT group but that of the control group was sharply decreased to 4.5% after 72 h of in vitro storage (4°C). The post-thawed sperm motility analysis showed that there was no significant difference between the MT+DMSO group and MT group, but the fertility rate in the MT group was significantly higher with a value of 42.30±2.57(%) than any other experimental groups, including the MT+DMSO group (P<0.05). Meanwhile, we also found that there was no significantly positive effect on post-thawed sperm motility with Y addition. Interestingly, although the results showed that the MT+DMSO group and MT group had similar effects on the post-thawed sperm motility, the MT+DMSO group had higher hatching rate compared to any other tested groups, including the MT group. Meanwhile, we also found that PG as cryoprotectant was unsuitable for sperm cryopreservation of Amur sturgeon. In conclusion, our results provides invaluable basis in further studies for the optimization technology of artificial propagation in Amur sturgeon.
Keywords
Acipenser schrenckii, Cryoprotectant, Sperm Cryopreservation, Embryo Development
To cite this article
Xiujuan Zhang, Jinge Ma, Linmiao Li, Haiying Jiang, Jinping Chen, Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (Acipenser schrenckii), Animal and Veterinary Sciences. Vol. 6, No. 4, 2018, pp. 51-57. doi: 10.11648/j.avs.20180604.11
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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