Volume 4, Issue 3, May 2016, Page: 39-46
Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin
Alemselam Birhanu Mekonnin, MRC Centre for Reproductive Health, The Queen’s Medical Research Institute College of Medicine and Veterinary Medicine, University of Edinburgh, Edinburgh, United Kingdom
A. Forbes Howie, MRC Centre for Reproductive Health, The Queen’s Medical Research Institute College of Medicine and Veterinary Medicine, University of Edinburgh, Edinburgh, United Kingdom
Christopher R. Harlow, MRC Centre for Reproductive Health, The Queen’s Medical Research Institute College of Medicine and Veterinary Medicine, University of Edinburgh, Edinburgh, United Kingdom
Goitom Gidey, College of Veterinary Medicine, Mekelle University, Mekelle, Ethiopia
Desalew Tadesse Tegegne, College of Veterinary Medicine, Mekelle University, Mekelle, Ethiopia
Gidena Desta, College of Veterinary Medicine, Mekelle University, Mekelle, Ethiopia
Tadesse Gugsa, Tigray Regional State Agriculture Bureau, Tigray, Mekelle, Ethiopia
Berihu Gebrekidan, College of Veterinary Medicine, Mekelle University, Mekelle, Ethiopia
Gebregiorgis Ashebir, College of Veterinary Medicine, Mekelle University, Mekelle, Ethiopia
Simon C. Riley, MRC Centre for Reproductive Health, The Queen’s Medical Research Institute College of Medicine and Veterinary Medicine, University of Edinburgh, Edinburgh, United Kingdom
Received: Dec. 13, 2015;       Accepted: Apr. 27, 2016;       Published: May 23, 2016
DOI: 10.11648/j.avs.20160403.12      View  5133      Downloads  288
Abstract
Simplifying and improving our understanding of the protocols for inducing or synchronizing estrus is important for improving management of dairy cattle. This study evaluated the estrus response and conception rate of crossbred (Holstein Friesian X Zebu) dairy cows (n=75) and heifers (n=47) assigned to a 10-day controlled internal drug release (CIDR) device – prostaglandin F2-alpha (PGF2α) – equine chorionic gonadotrophin (eCG) based estrus synchronization protocol. Animals were assigned to three groups (a) anestrus (n=62), (b) repeat-breeders (n=11) and (c) silent estrus (n=46), according to their reproductive history, per rectal palpation, and progesterone concentrations determined by On-Farm enzyme-linked immunosorbent assays (ELISA) in milk (lactating cows) or serum (dry cows and heifers). For each animal, a CIDR device (containing 1.38g progesterone) was inserted into the vagina and allowed to remain in-situ for 10 days. On Day 8 after device insertion, a dose of 500µg of PGF2α was administered intramuscularly (IM). On Day 10 (device removal), 500 IU of eCG was given IM. Animals were inseminated or mated 48 and 72 hrs post device removal. Pregnancy was assessed 20-24 days post insemination by measurement of progesterone in milk/serum and pregnancy was confirmed by per rectal palpation and/or ultrasonography 44-90 days post insemination. Overall estrus response and conception rates were 97.5% and 78.3%, respectively, with no significant differences in parity, pre-treatment reproductive status and farming system (smallholder vs organized commercial farms). Conception rate after induced/synchronized estrus was 82.9% and 72.1% in cows and heifers, respectively, with an overall calving rate of 94.4%. In conclusion, the present study has shown high estrus response and conception rate in crossbred dairy cattle using this CIDR - PGF2α - eCG based estrus synchronization protocol. Application of this protocol is highly recommended to enhance fertility of dairy cattle in the study area, and this could readily be applied to other regions.
Keywords
Cattle, CIDR, eCG, Estrus, Fertility, PGF2α, Synchronization
To cite this article
Alemselam Birhanu Mekonnin, A. Forbes Howie, Christopher R. Harlow, Goitom Gidey, Desalew Tadesse Tegegne, Gidena Desta, Tadesse Gugsa, Berihu Gebrekidan, Gebregiorgis Ashebir, Simon C. Riley, Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin, Animal and Veterinary Sciences. Vol. 4, No. 3, 2016, pp. 39-46. doi: 10.11648/j.avs.20160403.12
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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